Nucleic Acid Aptamers

Nucleic Acid Aptamers
Author: Günter Mayer
Publisher: Humana
Total Pages: 0
Release: 2015-11-10
Genre: Science
ISBN: 9781493931965

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

The Aptamer Handbook

The Aptamer Handbook
Author: Sven Klussmann
Publisher: John Wiley & Sons
Total Pages: 518
Release: 2006-08-21
Genre: Science
ISBN: 3527607919

In The Aptamer Handbook, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.

Aptamers for Medical Applications

Aptamers for Medical Applications
Author: Yiyang Dong
Publisher: Springer Nature
Total Pages: 470
Release: 2021-03-25
Genre: Science
ISBN: 9813348380

This book outlines comprehensively the main medical uses of aptamers, from diagnosis to therapeutics in fourteen chapters. Pioneering topics covered include aptamer pharmaceuticals, aptamers for malign tumors, aptamers for personalized therapeutics and aptamers for point-of-care testing. The book offers an essential guide for medical scientists interested in developing aptamer-based schemes for better theranostics. It is therefore of interest for not only academic researchers, but also practitioners and medical researchers in various fields of medical science, medical research and bio-analytical chemistry.

Aptamers

Aptamers
Author: Gulab Singh Yadav
Publisher: Springer Nature
Total Pages: 186
Release: 2019-11-11
Genre: Science
ISBN: 9811388369

The book discusses the basics of aptamers and the advent of aptamer-based technology in recent times. The book covers the diverse applications of aptamers, such as in detection of animal and plant pathogens, disease diagnosis and therapeutics, environmental contamination detection etc. Besides these applications, the book also describes the use of these synthetic or modified DNA, as drug delivery vehicles. The different chapters describe how the binding capacity and specificity of aptamers can be exploited in various ways. The book also discusses how these attributes of aptamers can outdo the antibody technology in biomedical and diagnostic solutions. This crisp and concise book gives the readers an insight into the most recent biotechnological applications of aptamers. ​

Aptamers in Bioanalysis

Aptamers in Bioanalysis
Author: M. Mascini
Publisher: John Wiley & Sons
Total Pages: 346
Release: 2009-04-27
Genre: Science
ISBN: 0470380764

This is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states. This is a core reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, biotechnologists, and others.

Aptamers

Aptamers
Author: Julian Alexander Tanner
Publisher: MDPI
Total Pages: 301
Release: 2018-08-27
Genre: Science
ISBN: 303897059X

This book is a printed edition of the Special Issue " Aptamers" that was published in IJMS

Aptamers

Aptamers
Author: Rakesh N. Veedu
Publisher: CRC Press
Total Pages: 209
Release: 2017-03-03
Genre: Medical
ISBN: 1315341123

Aptamers, often termed as ‘chemical antibodies,’ are an emerging class of synthetic ligands for efficient target-specific molecular recognition. The objective of this book is to highlight recent advances and potential of aptamers in various disease conditions. . This book focuses on the applications of aptamers in targeted nanotherapy, detection, and in molecular imaging in various disease conditions such as cancer, neurological diseases and infectious diseases.

Aptamers Engineered Nanocarriers for Cancer Therapy

Aptamers Engineered Nanocarriers for Cancer Therapy
Author: Prashant Kesharwani
Publisher: Woodhead Publishing
Total Pages: 558
Release: 2022-09-25
Genre: Technology & Engineering
ISBN: 0323855768

Aptamers Engineered Nanocarriers for Cancer Therapy details the selection technologies, biological characteristics, and clinical uses of aptamer-based nano agents for cancer therapeutics. The book helps facilitate speedy solutions for some of the problems pertaining to the manufacture of nano-aptamers – such as toxicity, thermal stability, cost efficiency, tumor penetration and blood stability. Key chapters cover cell-SELEX technology for aptamer selection, mechanisms of multi-drug resistance of cancer, the relevance of aptamers as anticancer therapies, as well as the broad range of aptamer-functionalized nanostructures available. This book provides exciting insights into this relatively new approach to cancer therapeutics, and will be of interest to materials scientists, biomedical engineers, molecular biologists, biochemists and clinical scientists, with a focus on cancer therapy. Reviews the mechanisms behind multi-drug resistance (MDR) in cancer and how aptamer-mediated novel therapeutic agents and strategies can facilitate MDR reversal Covers a range of aptamers engineered nanostructures, including PLGA nanoparticles, silica nanoparticles, quantum dots, nucleic acid aptamers, and more Discusses the challenges associated with using aptamers as cancer therapeutics and how this translates into clinical use

Aptamers for Food Applications

Aptamers for Food Applications
Author: Yiyang Dong
Publisher: Elsevier
Total Pages: 352
Release: 2023-05-27
Genre: Science
ISBN: 0323985149

Aptamers for Food Applications: Safety, Authenticity, and Integrity outlines the main applications of aptamers to ensure food safety, quality and compliance that are crucial for food science, public health and human sustainability. Aptamers or aptamer-based analytical devices are comprehensively summarized to tackle various food contamination or food adulteration problems. Topics covered in this book include aptamers for foodborne microbial pathogens, pesticide or veterinary drug residues, heavy metals, biotoxins, abused food additives, illegal food adulterants, POPs, GMOs, food allergens and food contact material migrants. It is therefore of interest for not only analytical investigators and food safety inspectors, but also regulatory practitioners and risk assessors in various food science and research fields. Presents the versatilities of aptamers for food safety, quality and compliance Provides utilities of aptamers for the analysis of almost all risk factors in foodstuffs Includes food quality control and regulatory measures such as HACCP, MRLs, proficiency test and uncertainty assessment, along with case illustrations

Aptamers for Analytical Applications

Aptamers for Analytical Applications
Author: Yiyang Dong
Publisher: John Wiley & Sons
Total Pages: 426
Release: 2019-02-11
Genre: Science
ISBN: 3527342672

An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.