Application Of The Orbitrap Mass Analyzer To Targeted Proteomics And Gas Chromatography Mass Spectrometry Of Small Molecules
Download Application Of The Orbitrap Mass Analyzer To Targeted Proteomics And Gas Chromatography Mass Spectrometry Of Small Molecules full books in PDF, epub, and Kindle. Read online free Application Of The Orbitrap Mass Analyzer To Targeted Proteomics And Gas Chromatography Mass Spectrometry Of Small Molecules ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
| Author | : |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2012 |
| Genre | : |
| ISBN | : |
The work described herein spans two disparate topics: 1) the use of a high resolution and accurate mass analyzer to facilitate targeted proteomic methods, and 2) the development and applications of novel instrumentation for high resolution gas chromatography/mass spectrometry. While disparate, a common theme of a high resolution and accurate mass analyzer, the Orbitrap, ties the work together into, broadly, a treatise on the benefits of high resolution and accurate mass measurements in mass spectrometric research. In Chapter 1, I present an introduction on the Orbitrap mass analyzer and motivate the need for high resolution and accurate mass measurements in mass spectrometry. I will discuss the theory of Orbitrap operation, as well as the practical factors that may enhance or limit its performance. In Chapter 2, I delve into the field of targeted proteomics with the introduction of the "parallel reaction monitoring" (PRM) analysis paradigm using a bench-top quadrupole-Orbitrap LC/MS. While targeted proteomics is usually the domain of low resolution but highly sensitive mass analyzers, I demonstrate the utility of high resolution with a direct comparison to the "gold-standard" triple-quadrupole MS-based selected reaction monitoring (SRM) approach. This PRM approach has already generated much interest in the proteomics community since its publication. In Chapters 3 through 5, I transition to GC/MS, another field dominated by low resolution and fast scanning mass analyzers. In Chapter 3, I detail the construction and characterization of a proof-of-principle instrument for high resolution GC/MS built using an electron transfer dissociation (ETD)-enabled quadrupole linear ion trap (QLT)-Orbitrap MS. While the performance of this instrument was hindered by its design, the benefits of the Orbitrap for unambiguous elemental composition assignment to small molecules were clear, and further development was deemed warranted. In Chapter 4, I detail that further development in the design and construction of a dedicated, applications-grade bench-top quadrupole-Orbitrap GC/MS. The application of this instrument to the non-targeted characterization of unknown metabolites is presented in Chapter 5. Additionally in Chapter 5, I introduce a new method for data-dependent acquisition termed "molecular-ion directed acquisition", which aims to maximize the information content from non-targeted tandem MS in metabolomic studies
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 526 |
| Release | : 2016-06-02 |
| Genre | : Law |
| ISBN | : 0444635734 |
Applications of Time-of-Flight and Orbitrap Mass Spectrometry in Environmental, Food, Doping, and Forensic Analysis deals with the use of high-resolution mass spectrometry (MS) in the analysis of small organic molecules. Over the past few years, time-of-flight (ToF) and Orbitrap MS have both experienced tremendous growth in a great number of analytical sectors and are now well established in many laboratories where high requirements are placed on analytical performance. This book gives a head-to-head comparison of these two technologies that compete directly with each other. As users with hands-on experience in both techniques, the authors provide a balanced description of the strengths and weaknesses of both techniques. In the vast majority of cases, ToF-MS and Orbitrap-MS have been used for qualitative purposes, mainly identification of discrete molecular entities such as drug metabolites or transformation products of environmental contaminants. This paradigm is now changing as quantitative capabilities are increasingly being explored, as are non-target approaches for unbiased broad-scope screening. In view of the continuous innovation of high-resolution MS instrument manufacturers in designing and developing more powerful machines, technological advances in both hardware and software are considerable, with many novel applications. This book summarizes and analyzes these trends. The compilation of selected examples from diverse analytical fields will allow the readers to discover not only the potential of high-resolution MS in their sector, but also shows advances in other fields that rely on hi-res MS. Provides comprehensive coverage of applications of time-of-flight and orbitrap mass spectrometry in environmental, food, doping, and forensic analysis Explores a variety of specialized techniques, giving a balanced description of the strengths and weaknesses of each Presents a general overview of imaging techniques within analysis
| Author | : John C. Lindon |
| Publisher | : Elsevier |
| Total Pages | : 622 |
| Release | : 2018-10-04 |
| Genre | : Science |
| ISBN | : 0128122943 |
The Handbook of Metabolic Phenotyping is the definitive work on the rapidly developing subject of metabolic phenotyping. It explores in detail the wide array of analytical chemistry and statistical modeling techniques used in the field, coupled with surveys of the various application areas in human development, nutrition, disease, therapy, and epidemiology to create a comprehensive exploration of the area of study. It covers recent studies that integrate the various -omics data sets to derive a systems biology view. It also addresses current issues on standardization, assay and statistics validation, and data storage and sharing. Written by experts with many years of practice in the field who pioneered many of the approaches widely used today, The Handbook of Metabolic Phenotyping is a valuable resource for postgrads and research scientists studying and furthering the field of metabolomics. Contains theoretical and practical explanations of all the main analytical chemistry techniques used in metabolic phenotyping Explores, in detail, the many diverse statistical approaches used in the field Offers practical tips for successfully conducting metabolic phenotyping studies Features reviews of all of the various fields of activity relating to human studies
| Author | : Nader Rifai |
| Publisher | : Elsevier |
| Total Pages | : 218 |
| Release | : 2018-06-26 |
| Genre | : Science |
| ISBN | : 0128160640 |
Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups. Provides concrete examples of the main applications of mass spectrometry technology Describes current capabilities of the LC- and MS-based analytical methods Details methods for successful analytical work in the field
| Author | : Mahmood Aliofkhazraei |
| Publisher | : BoD – Books on Demand |
| Total Pages | : 284 |
| Release | : 2017-06-07 |
| Genre | : Science |
| ISBN | : 9535132237 |
Mass spectrometry is an analytical technique that can be used for the structural characterization and quantification of a wide range of molecules. The technique is extensively used by chemists for the analysis of small and volatile organic compounds. Mass spectrometry has long been an important technique for the identification of materials ranging from pure compounds to complex mixtures. Mass spectrometry can be used to determine molecular weight of compounds or using different ionization conditions, can provide more structural details through the analysis of fragmentation patterns. This level of detail can be attained for pure compounds and some mixtures. Mass spectrometry can also be combined with separation techniques such as gas chromatography or liquid chromatography to allow more complex mixtures to be examined. These hyphenated techniques provide a range of options for the characterization of complex materials.
| Author | : Raymond E. March |
| Publisher | : CRC Press |
| Total Pages | : 952 |
| Release | : 2010-05-25 |
| Genre | : Science |
| ISBN | : 1420083724 |
Reflecting the substantial increase in popularity of quadrupole ion traps and Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers, Practical Aspects of Trapped Ion Mass Spectrometry, Volume IV: Theory and Instrumentation explores the historical origins of the latest advances in this expanding field. It covers new methods for trapp
| Author | : James McCullagh |
| Publisher | : |
| Total Pages | : 193 |
| Release | : 2019-06-13 |
| Genre | : |
| ISBN | : 0198789041 |
The renowned Oxford Chemistry Primers series, which provides focused introductions to a range of important topics in chemistry, has been refreshed and updated to suit the needs of today's students, lecturers, and postgraduate researchers. The rigorous, yet accessible, treatment of each subjectarea is ideal for those wanting a primer in a given topic to prepare them for more advanced study or research. Moreover, cutting-edge examples and applications throughout the texts show the relevance of the chemistry being described to current research and industry.The learning features provided, including questions at the end of every chapter and online multiple-choice questions, encourage active learning and promote understanding. Furthermore, frequent diagrams, margin notes, further reading, and glossary definitions all help to enhance a student'sunderstanding of these essential areas of chemistry.This brand new addition to the series provides the most concise, clear, and accessible first introduction to the basic principles of mass spectrometry.Online resourcesThe online resources that accompany Mass Spectrometry include:For students:- Multiple-choice questions for self-directed learningFor registered adopters of the text:- Figures from the book available to download
| Author | : Oscar Alzate |
| Publisher | : CRC Press |
| Total Pages | : 356 |
| Release | : 2009-10-26 |
| Genre | : Medical |
| ISBN | : 1420076264 |
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
| Author | : Jeroen Kool |
| Publisher | : John Wiley & Sons |
| Total Pages | : 402 |
| Release | : 2015-05-04 |
| Genre | : Science |
| ISBN | : 3527334645 |
This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
| Author | : Roberto Romero-González |
| Publisher | : Elsevier |
| Total Pages | : 362 |
| Release | : 2017-03-07 |
| Genre | : Science |
| ISBN | : 0128096489 |
Applications of High Resolution Mass Spectrometry: Food Safety and Pesticide Residue Analysis is the first book to offer complete coverage of all aspects of high resolution mass spectrometry (HRMS) used for the analysis of pesticide residue in food. Aimed at researchers and graduate students in food safety, toxicology, and analytical chemistry, the book equips readers with foundational knowledge of HRMS, including established and state-of-the-art principles and analysis strategies. Additionally, it provides a roadmap for implementation, including discussions of the latest instrumentation and software available. Detailed coverage is given to the application of HRMS coupled to ultra high-performance liquid chromatography (UHPLC-HRMS) in the analysis of pesticide residue in fruits and vegetables and food from animal origin. The book also discusses extraction procedures and the challenges of sample preparation, gas chromatography coupled to high resolution mass spectrometry, flow injection-HRMS, ambient ionization, and identification of pesticide transformation products in food. Responding to the fast development and application of these new procedures, this book is an essential resource in the food safety field. Arms researchers with an in-depth resource devoted to the rapid advances in HRMS tools and strategies for pesticide residue analysis in food Provides a complete overview of analytical methodologies and applications of HRMS, including UHPLC-HRMS, HRMS coupled with time of flight (TOF) and/or GC-Orbitrap, and flow injection-HRMS Discusses the current international regulations and legislation related to the use of HRMS in pesticide residue analysis Features a chapter on the hardware and software available for HRMS implementation Offers separate chapters on HRMS applied to pesticide residue analysis in fruits and vegetables and in food from animal origin
