Analytical and Preparative Separation Methods of Biomacromolecules

Analytical and Preparative Separation Methods of Biomacromolecules
Author: Hassan Y. Aboul-Enein
Publisher: CRC Press
Total Pages: 482
Release: 2020-10-15
Genre: Science
ISBN: 1000146251

Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Analytical and Preparative Separation Methods of Biomacromolecules

Analytical and Preparative Separation Methods of Biomacromolecules
Author: Hassan Y. Aboul-Enein
Publisher: CRC Press
Total Pages: 479
Release: 2020-10-14
Genre: Science
ISBN: 1000110400

Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Encyclopedia of Analytical Science

Encyclopedia of Analytical Science
Author:
Publisher: Elsevier
Total Pages: 5142
Release: 2019-04-02
Genre: Science
ISBN: 008101984X

The third edition of the Encyclopedia of Analytical Science, Ten Volume Set is a definitive collection of articles covering the latest technologies in application areas such as medicine, environmental science, food science and geology. Meticulously organized, clearly written and fully interdisciplinary, the Encyclopedia of Analytical Science, Ten Volume Set provides foundational knowledge across the scope of modern analytical chemistry, linking fundamental topics with the latest methodologies. Articles will cover three broad areas: analytical techniques (e.g., mass spectrometry, liquid chromatography, atomic spectrometry); areas of application (e.g., forensic, environmental and clinical); and analytes (e.g., arsenic, nucleic acids and polycyclic aromatic hydrocarbons), providing a one-stop resource for analytical scientists. Offers readers a one-stop resource with access to information across the entire scope of modern analytical science Presents articles split into three broad areas: analytical techniques, areas of application and and analytes, creating an ideal resource for students, researchers and professionals Provides concise and accessible information that is ideal for non-specialists and readers from undergraduate levels and higher

Methods for Affinity-Based Separations of Enzymes and Proteins

Methods for Affinity-Based Separations of Enzymes and Proteins
Author: Munishwar N. Gupta
Publisher: Birkhäuser
Total Pages: 234
Release: 2013-12-01
Genre: Science
ISBN: 3034881274

One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.

Bioaffinity Chromatography

Bioaffinity Chromatography
Author: J. Turková
Publisher: Elsevier
Total Pages: 819
Release: 1993-09-14
Genre: Science
ISBN: 0080858635

Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography.Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.

Separation Techniques in Clinical Chemistry

Separation Techniques in Clinical Chemistry
Author: Hassan Y. Aboul-Enein
Publisher: CRC Press
Total Pages: 548
Release: 2003-05-28
Genre: Science
ISBN: 9780203911105

This reference examines innovations in separation science for improved sensitivity and cost-efficiency, increased speed, higher sample throughput and lower solvent consumption in the assessment, evaluation, and validation of emerging drug compounds. It investigates breakthroughs in sample pretreatment, HPLC, mass spectrometry, capillary electrophor

Electrochemical Sensors in Bioanalysis

Electrochemical Sensors in Bioanalysis
Author: Raluca-Ioana Stefan
Publisher: CRC Press
Total Pages: 320
Release: 2001-08-15
Genre: Technology & Engineering
ISBN: 9780824706623

"Covers the most recent methods and materials for the construction, validation, analysis, and design of electrochemical sensors for bioanalytical, clinical, and pharmaceutical applications--emphasizing the latest classes of enantioselective electrochemical sensors as well as electrochemical sensors for in vivo and in vitro diagnosis, for DNA assay and HIV detection, and as detectors in flow systems. Contains current techniques for the assay or biochemical assay of biological fluids and pharmaceutical compounds."

Proteomics and Peptidomics

Proteomics and Peptidomics
Author: Gyorgy Marko-Varga
Publisher: Elsevier
Total Pages: 663
Release: 2005-12-23
Genre: Language Arts & Disciplines
ISBN: 044451810X

Proteomics and peptidomics is the detailed understanding of the role that proteins and peptides play in health and disease and is a necessary compliment to genetic analysis. The functional expression analysis of both proteins and peptides plays a central role in modern drug discovery as well as drug development, and is also a key research area in systems biology. Proteomics and Peptidomics captures the width as well as the depth within the area and exemplifies the variety as well as the traditional basis of analytical chemistry that is needed in order to move forward in expression analysis studies. As a fast emerging field, it gives and overview of parts within the field combined with highly specialized and dedicated topics that are intended to compliment each other.

Encyclopedia of Chromatography 2004 Update Supplement

Encyclopedia of Chromatography 2004 Update Supplement
Author: Jack Cazes
Publisher: CRC Press
Total Pages: 602
Release: 2004-08-11
Genre: Science
ISBN: 1482298570

In step with novel technologies and methodologies that have reshaped chromatography in recent years, this supplement reviews developments in HPLC, TLC, SFC, CCC, and other areas-presenting 50 authoritative entries filled with practical information vital to applications from biotechnology to environmental science to clinical pathology.

Isolation and Purification of Proteins

Isolation and Purification of Proteins
Author: Rajni Hatti-Kaul
Publisher: CRC Press
Total Pages: 780
Release: 2003-02-05
Genre: Medical
ISBN: 1135545243

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host