Advances In Glycobiotechnology
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| Author | : Erdmann Rapp |
| Publisher | : Springer Nature |
| Total Pages | : 467 |
| Release | : 2021-07-27 |
| Genre | : Science |
| ISBN | : 3030695905 |
This book reviews the latest trends in glycobiotechnology, it offers an authoritative discussion about future directions of glycoengineering, and it provides a comprehensive overview about the current and emerging approaches to identify, quantify and characterize glycosylated proteins. Divided into 14 chapters, the book outlines recombinant glycoprotein expression in mammalian cells, insect cells, yeast, and bacterial systems. It covers the chemical and enzymatic syntheses of glycans and glyconjugates, and addresses the impact of glycosylation on protein function for the development of biologicals including vaccines. In the final chapters of the book, readers will discover more about the state-of-the-art in glycomics, glycoproteomics and glycan array technologies.
| Author | : Vijai Kumar Gupta |
| Publisher | : John Wiley & Sons |
| Total Pages | : 742 |
| Release | : 2015-04-20 |
| Genre | : Technology & Engineering |
| ISBN | : 1118733495 |
Bioactive compounds play a central role in high-value product development in the chemical industry. Bioactive compounds have been identified from diverse sources and their therapeutic benefits, nutritional value and protective effects in human and animal healthcare have underpinned their application as pharmaceuticals and functional food ingredients. The orderly study of biologically active products and the exploration of potential biological activities of these secondary metabolites, including their clinical applications, standardization, quality control, mode of action and potential biomolecular interactions, has emerged as one of the most exciting developments in modern natural medicine. Biotechnology of Bioactive Compounds describes the current stage of knowledge on the production of bioactive compounds from microbial, algal and vegetable sources. In addition, the molecular approach for screening bioactive compounds is also discussed, as well as examples of applications of these compounds on human health. The first half of the book comprises information on diverse sources of bioactive compounds, ranging from microorganisms and algae to plants and dietary foods. The second half of the book reviews synthetic approaches, as well as selected bioactivities and biotechnological and biomedical potential. The bioactive compounds profiled include compounds such as C-phycocyanins, glycosides, phytosterols and natural steroids. An overview of the usage of bioactive compounds as antioxidants and anti-inflammatory agents, anti-allergic compounds and in stem cell research is also presented, along with an overview of the medicinal applications of plant-derived compounds. Biotechnology of Bioactive Compounds will be an informative text for undergraduate and graduate students of bio-medicinal chemistry who are keen to explore the potential of bioactive natural products. It also provides useful information for scientists working in various research fields where natural products have a primary role.
| Author | : Deepti Yadav |
| Publisher | : Elsevier |
| Total Pages | : 307 |
| Release | : 2024-09-10 |
| Genre | : Science |
| ISBN | : 032398584X |
Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins. - Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systems - Can also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detail - Consists of notes on troubleshooting from the eminent researchers in the field - Provides comprehensive information on protein production, purification and characterization in a single volume - Describes different purification methods for comparatively difficult to obtain proteins - Brings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins
| Author | : Chittaranjan Kole |
| Publisher | : Springer Nature |
| Total Pages | : 422 |
| Release | : 2023-11-22 |
| Genre | : Science |
| ISBN | : 9819948592 |
This edited book is an in-depth compilation of recent tools and techniques, concepts and strategies used globally in plant molecular farming (PMF) for the cost-effective bulk production of recombinant proteins, secondary metabolites, and other biomolecules. The book presents an overview of success stories of PMF applications from developing countries to address poverty, achieve zero hunger, good health and well-being, thus achieving the UN SDGs 1, 2, and 3. The book deep dives into recent extraction and downstream processing methodologies, its co-existence with conventional agriculture, global governance and finally opportunities, challenges, and future perspectives in plant molecular farming. It focuses on plastid/chloroplast transformation (transplastomics) and its application in plant molecular farming. The books highlight recent advances in genome editing, synthetic biology, glycosylation and glyco-engineering for improved plant molecular farming by marker-free and tissue-specific systems via cisgenic and transgenic crops. In depth discussions on biosafety issues and bio-containment strategies have also been included. The book has 15 chapters authored by globally leading experts on the subject, presenting opportunities & challenges for bio-industrial researchers and entrepreneurs. It is useful to researchers, industrialists, entrepreneurs, policy planners, academician, and students across the disciplines.
| Author | : Vijai G. Gupta |
| Publisher | : Newnes |
| Total Pages | : 513 |
| Release | : 2013-12-05 |
| Genre | : Technology & Engineering |
| ISBN | : 0444595643 |
Bioenergy Research: Advances and Applications brings biology and engineering together to address the challenges of future energy needs. The book consolidates the most recent research on current technologies, concepts, and commercial developments in various types of widely used biofuels and integrated biorefineries, across the disciplines of biochemistry, biotechnology, phytology, and microbiology. All the chapters in the book are derived from international scientific experts in their respective research areas. They provide you with clear and concise information on both standard and more recent bioenergy production methods, including hydrolysis and microbial fermentation. Chapters are also designed to facilitate early stage researchers, and enables you to easily grasp the concepts, methodologies and application of bioenergy technologies. Each chapter in the book describes the merits and drawbacks of each technology as well as its usefulness. The book provides information on recent approaches to graduates, post-graduates, researchers and practitioners studying and working in field of the bioenergy. It is an invaluable information resource on biomass-based biofuels for fundamental and applied research, catering to researchers in the areas of bio-hydrogen, bioethanol, bio-methane and biorefineries, and the use of microbial processes in the conversion of biomass into biofuels. - Reviews all existing and promising technologies for production of advanced biofuels in addition to bioenergy policies and research funding - Cutting-edge research concepts for biofuels production using biological and biochemical routes, including microbial fuel cells - Includes production methods and conversion processes for all types of biofuels, including bioethanol and biohydrogen, and outlines the pros and cons of each
| Author | : Alexandra Castilho |
| Publisher | : Humana |
| Total Pages | : 0 |
| Release | : 2015-06-17 |
| Genre | : Science |
| ISBN | : 9781493927593 |
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.
| Author | : Bhim Pratap Singh |
| Publisher | : Springer |
| Total Pages | : 370 |
| Release | : 2017-01-21 |
| Genre | : Science |
| ISBN | : 3319341065 |
The Kingdom fungi encompass a massive diversity of taxa with wide-ranging ecologies, life cycles, and morphologies ranging from unicellular aquatic chytrids to large mushrooms. Before molecular methods came in existence, taxonomists considered this Kingdom to be a member of the plant kingdom due to certain life styles like immobility and growth habitats. Molecular markers (also known as DNA markers), facilitated a better alternative method over traditional morphological methods, employed for the identification, characterization, and to understand the evolution of fungi. The morphological methods used for identification are mainly dependent on spore color or microscopic features whereas molecular markers are based on DNA polymorphism in the genomic organization. Phylogenetic studies reported in last decade, based on molecular markers, have reshaped the classification system of Kingdom fungi, which divided into one subkingdom, seven phyla, and ten subphyla. Recent advances in molecular mycology have opened the way for researchers to identify and characterize novel fungal species from unique environments. Mycology is concerned with the systematic study of fungi, including their genetic and biochemical properties, their use to humans as a source of medicine and food, as well as their dangers, such as poisoning and infections. In the 21st century with the development of DNA sequencing technologies and phylogenetic analysis based on molecular markers, new insights into fungal taxonomy were provided. This book contains a thorough discussion of molecular characterization and detection of different groups of fungi by using PCR-based markers and provides a comprehensive view of the applications and uses of different molecular markers in molecular mycology. It also addresses the recent molecular markers employed to solve the problems of identification and discusses current approaches used in molecular characterization and detection of fungi.
| Author | : Indu Ravi |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 264 |
| Release | : 2013-10-21 |
| Genre | : Science |
| ISBN | : 8132215540 |
The book “Advances in Biotechnology” is about recent advances in some of the important fields that are ongoing in certain biotechnological applications. Biotechnology has been quite helpful in keeping pace with the demands of every increasing human population and in improving the quality of human life. Major biotechnological achievements associated with human welfare have been from the fields like genetic engineering; transgenic plants and animals; genomics, proteomics, monoclonal antibodies for the diagnosis of disease, gene therapy etc. Fourteen authoritative chapters written by experts having experience in academics and research on current developments and future trends in biotechnology have been empathized. The book provides a detailed account of various methodologies used in biotechnology i.e. High capacity vectors, DNA sequencing dealing with next generation sequencing, Molecular markers, DNA microarray technology, as well as Proteomics that have revolutionized biotechnology with a wide array of applications. The book not only presents a well-founded explanation of the topics but also aims to present up-to-date reviews of current research efforts, some thoughtful discussions on the potential benefits and risks involved in producing biotechnological products and the challenges of bringing such products to market. It will prove to be an excellent reference work for both academicians and researchers, indicating new starting points to young researchers for new projects in the field. The book is intended for biotechnologist, biologist, researchers, teachers and students of Biosciences and Biotechnology.
| Author | : Els J. M. Van Damme |
| Publisher | : Frontiers Media SA |
| Total Pages | : 137 |
| Release | : 2015-02-12 |
| Genre | : Botany |
| ISBN | : 2889194213 |
Plants synthesize a wide variety of unique glycan structures which play essential roles during the life cycle of the plant. Being omnipresent throughout the plant kingdom, ranging from simple green algae to modern flowering plants, glycans contribute to many diverse processes. Glycans can function as structural components in the plant cell wall, assist in the folding of nascent proteins, act as signaling molecules in plant defense responses or (ER) stress pathways, or serve within the energy metabolism of a plant. In most cases, glycans are attached to other macromolecules to form so-called glycoconjugates (e.g. glycoproteins, proteoglycans and glycolipids), but they can also be present as free entities residing in the plant cell. Next to the broad, complex set of glycans, plants also evolved an elaborate collection of lectins or proteins with a lectin-like domain, which can recognize and bind to endogenous (plants-own) or exogenous (foreign) glycans. Though still poorly understood in plants, the dynamic interactions between lectins and carbohydrate structures are suggested to be involved in gene transcription, protein folding, protein transport, cell adhesion, signaling as well as defense responses. As such, a complex and largely undetermined glycan-interactome is established inside plant cells, between cells and their surrounding matrix, inside the extracellular matrix, and even between organisms. Studying the biological roles of plant glycans will enable to better understand plant development and physiology in order to fully exploit plants for food, feed and production of pharmaceutical proteins. In this Research Topic, we want to provide a platform for articles describing the latest research, perspectives and methodologies related to the fascinating world of plant glycobiology, with a focus on following subjects: 1. Identification and characterization of plant glycans, their biosynthetic and degradation enzymes 2. Characterization of plant lectins and glycoproteins 3. Plant glycans in the plant’s energy metabolism 4. Role of plant glycans in plant defense signaling 5. Use of plant lectins in pest control 6. Plant lectins as new tools in human medicine 7. Glyco-engineering in plants
| Author | : Dhananjaya Pratap Singh |
| Publisher | : Springer |
| Total Pages | : 354 |
| Release | : 2016-02-22 |
| Genre | : Science |
| ISBN | : 813222647X |
How to achieve sustainable agricultural production without compromising environmental quality, agro-ecosystem function and biodiversity is a serious consideration in current agricultural practices. Farming systems’ growing dependency on chemical inputs (fertilizers, pesticides, nutrients etc.) poses serious threats with regard to crop productivity, soil fertility, the nutritional value of farm produce, management of pests and diseases, agro-ecosystem well-being, and health issues for humans and animals. At the same time, microbial inoculants in the form of biofertilizers, plant growth promoters, biopesticides, soil health managers, etc. have gained considerable attention among researchers, agriculturists, farmers and policy makers. The first volume of the book Microbial Inoculants in Sustainable Agricultural Productivity - Research Perspectives highlights the efforts of global experts with regard to various aspects of microbial inoculants. Emphasis is placed on recent advances in microbiological techniques for the isolation, characterization, identification and evaluation of functional properties using biochemical and molecular tools. The taxonomic characterization of agriculturally important microorganisms is documented, along with their applications in field conditions. The book exploresthe identification, characterization and diversity analysis of endophytic microorganisms in various crops including legumes/ non-legumes, as well as the assessment of their beneficial impacts in the context of promotingplant growth. Moreover, it provides essential updates onthe diversity and role of plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal mycorrhizal fungi (AMF). Further chaptersexamine in detailbiopesticides, thehigh-density cultivation of bioinoculants in submerged culture, seed biopriming strategies for abiotic and biotic stress tolerance, andPGPR as abio-control agent. Given its content,the book offers a valuable resource for researchers involved in research and development concerningPGPR, biopesticides and microbial inoculants.
