CRISPR and RNAi Systems

CRISPR and RNAi Systems
Author: Kamel A Abd-Elsalam
Publisher: Elsevier
Total Pages: 842
Release: 2021-02-27
Genre: Technology & Engineering
ISBN: 0128219114

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. - Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection - Outlines the major techniques and applications of both CRISPR and RNAi technologies - Assesses the major challenges of escalating these technologies on a mass scale

RNAi Technology

RNAi Technology
Author: R. K. Gaur
Publisher: CRC Press
Total Pages: 388
Release: 2016-04-19
Genre: Science
ISBN: 1439873259

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

Advancement in Crop Improvement Techniques

Advancement in Crop Improvement Techniques
Author: Narendra Tuteja
Publisher: Woodhead Publishing
Total Pages: 444
Release: 2020-06-13
Genre: Technology & Engineering
ISBN: 0128185821

Advancement in Crop Improvement Techniques presents updates on biotechnology and molecular biological approaches which have contributed significantly to crop improvement. The book discusses the emerging importance of bioinformatics in analyzing the vast resources of information regarding crop improvement and its practical application and utilization. Throughout this comprehensive resource, emphasis is placed on various techniques used to improve agricultural crops, providing a common platform for the utility of these techniques and their combinations. Written by an international team of contributors, this book provides an in-depth analysis of existing tools and a framework for new research. - Reviews techniques used for crop improvement, from selection and crossing over, to microorganismal approaches - Explores the role of conventional biotechnology in crop improvement - Summarizes the combined approaches of cytogenetics and biotechnology for crop improvement, including the importance of molecular techniques in this process - Focuses on the emerging role of bioinformatics for crop improvement

Genetically Engineered Crops

Genetically Engineered Crops
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
Total Pages: 607
Release: 2017-01-28
Genre: Science
ISBN: 0309437385

Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.

Modulating Gene Expression

Modulating Gene Expression
Author: Aditi Singh
Publisher: BoD – Books on Demand
Total Pages: 136
Release: 2019-05-29
Genre: Medical
ISBN: 1838806962

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

Integrating Advanced High-throughput Technologies to Improve Plant Resilience to Environmental Challenges

Integrating Advanced High-throughput Technologies to Improve Plant Resilience to Environmental Challenges
Author: Freddy Mora-Poblete
Publisher: Frontiers Media SA
Total Pages: 179
Release: 2023-06-28
Genre: Science
ISBN: 2832527000

High-throughput technologies are those that allow the generation of large-scale data related to omic analysis (such as genomics, transcriptomics, proteomics, phenomics, and metabolomics). Currently, the development and use of these technologies has gone hand-in-hand with the development of bioinformatics tools, which have allowed the integration of omics-based data to understand crucial knowledge about the molecular systems that underlie the various functions of the plants. In recent years, there has been a huge development in the field of high-throughput biology, which is attributed to an era of tech startups. Genomics studies in plants, for instance, have benefited from advances in high-throughput technologies, such as high-throughput genotyping, which have allowed us to investigate the regulation and function of thousands of genes and genomic regions involved in a novel adaptation. In fact, with current high-throughput technologies, it is possible to recover substantial portions of the plant diversity, which is a key input for genetic improvement programs, food security and conservation programs. The integration of multilevel biological data, involving investigations at the molecular level (in genomics, transcriptomics, proteomics, and metabolomics, among others), have allowed more comprehensive studies, which broadens the opportunities to clarify key molecular aspects involved in the physiological responses to abiotic stresses.

Encyclopedia of Entomology

Encyclopedia of Entomology
Author: John L. Capinera
Publisher: Springer Science & Business Media
Total Pages: 1062
Release: 2008-08-11
Genre: Science
ISBN:

Bringing together the expertise of over 450 distinguished entomologists from 40 countries, this exhaustive work provides a global overview of insects and their close relatives. It is designed as an introduction to this fascinating group of animals.