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| Author | : Kenneth W. Adolph |
| Publisher | : CRC Press |
| Total Pages | : 488 |
| Release | : 1991-03-28 |
| Genre | : Science |
| ISBN | : 9780824784300 |
Contributors detail up-to-date guidelines for using molecular techniques, cytogenetic and linkage analysis, and cellular methods, emphasizing human cells and medically relevant research. They present results of recent applications of techniques and step-by-step protocols for cloning large DNA molecu
| Author | : Adrian Thomas Sumner |
| Publisher | : Springer |
| Total Pages | : 456 |
| Release | : 1990-11-22 |
| Genre | : Juvenile Nonfiction |
| ISBN | : |
| Author | : Arun Sharma |
| Publisher | : Butterworth-Heinemann |
| Total Pages | : 724 |
| Release | : 2014-04-24 |
| Genre | : Science |
| ISBN | : 1483100847 |
Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.
| Author | : Eisuke Gotoh |
| Publisher | : Springer Nature |
| Total Pages | : 195 |
| Release | : 2022-09-06 |
| Genre | : Science |
| ISBN | : 1071624334 |
This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
| Author | : Claudia Behrend |
| Publisher | : Springer |
| Total Pages | : 210 |
| Release | : 2017-07-06 |
| Genre | : Medical |
| ISBN | : 3319540998 |
This atlas presents the results of cases of structural chromosome aberrations based on the currently available methods of analysis for different types of abnormality. It particularly focuses on which spectrum should be chosen when combining the different techniques to achieve the best method of diagnosis in individual cases, for example direct preparation of cells and mitoses, short or long-time cell culture, fluorescence in situ hybridization (FISH), analysis of interphases, microarray or DNA sequencing. Generally it has to be taken into account, that the development of new and improved investigation methods is forthcoming. Thus, by improvement of diagnostic possibilities new fields of investigation arise, and special groups of patients with cytogenetic analyses can be re-analysed under new research questions.
| Author | : Torbjoern Caspersson |
| Publisher | : Elsevier |
| Total Pages | : 357 |
| Release | : 1973-01-01 |
| Genre | : Science |
| ISBN | : 0323162673 |
Chromosome Identification—Technique and Applications in Biology and Medicine contains the proceedings of the Twenty-Third Nobel Symposium held at the Royal Swedish Academy of Sciences in Stockholm, Sweden, on September 25-27,1972. The papers review advances in chromosome banding techniques and their applications in biology and medicine. Techniques for the study of pattern constancy and for rapid karyotype analysis are discussed, along with cytological procedures; karyotypes in different organisms; somatic cell hybridization; and chemical composition of chromosomes. This book is comprised of 51 chapters divided into nine sections and begins with a survey of the cytological procedures, including fluorescence banding techniques, constitutive heterochromatin (C-band) technique, and Giemsa banding technique. The following chapters explore computerized statistical analysis of banding pattern; the use of distribution functions to describe integrated profiles of human chromosomes; the uniqueness of the human karyotype; and the application of somatic cell hybridization to the study of gene linkage and complementation. The mechanisms for certain chromosome aberration are also analyzed, together with fluorescent banding agents and differential staining of human chromosomes after oxidation treatment. This monograph will be of interest to practitioners in the fields of biology and medicine.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 128 |
| Release | : 1988-01-01 |
| Genre | : Science |
| ISBN | : 0309038405 |
There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.
| Author | : Kiichi Fukui |
| Publisher | : CRC Press |
| Total Pages | : 304 |
| Release | : 2008 |
| Genre | : Science |
| ISBN | : |
Reviewing trends and techniques in current and future chromosome research, this volume draws upon scientists from many disciplines to introduce various nano devices and applications that have been developed for handling the smallest biological materials, especially chromosomes. It details the visualization of chromosomes at nano and micro levels, including the most advanced applications ever employed for Scanning Probe Microscopy. It also discusses the development of a sustainable chromosome imaging database; examines isolation, comparative proteome analyses, and structural/functional analyses including a new 3-dimensional model; and describes a new antibody production method for chromosomal proteins.
| Author | : Harry Harris |
| Publisher | : Plenum Publishing Corporation |
| Total Pages | : 352 |
| Release | : 1970 |
| Genre | : Science |
| ISBN | : 9780306396014 |
From reviews of previous volumes in the series: 'Extremely valuable...thoroughly recommended.'-Annals of Human Genetics 'The most lucid and stimulating discussions of the topic to be found anywhere.'-American Scientist
| Author | : Kiichi Fukui |
| Publisher | : CRC Press |
| Total Pages | : 298 |
| Release | : 1996-09-26 |
| Genre | : Science |
| ISBN | : 9780849389191 |
Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies. Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.
