The effects of transverse strain on an initially two-dimensional turbulent boundary layer are studied in a direct numerical simulation of a planar channel flow with impulsively started transverse pressure gradient. Consistent with experiments in three-dimensional boundary layers, the simulation shows a decrease in the Reynolds shear stress with increasing transverse strain. Also, the directions of the Reynolds shear stress vector and the mean velocity gradient vector were found to differ. In addition, the simulation shows a drop in the turbulent kinetic energy. Terms in the Reynolds stress transport equations were computed. The balances indicate that the decrease in turbulent kinetic energy is a result of a decrease in turbulence production, along with an increase in turbulent dissipation. The effects of the transverse pressure gradient on the instantaneous flow structures were investigated.