The Florida Passenger Rail System Study examined all existing and planned passenger rail systems in Florida that are under the jurisdiction of the Federal Railroad Administration. The study includes three components: a detailed inventory and description of the Florida Passenger Rail System, an analysis of incident data involving passenger rail operations and grade crossings, and an overview of jurisdictions that regulate passenger rail operations on a federal, state, regional and local level. Based on the findings of the three parts of the study, this report introduces a set of recommendations to improve passenger rail operations, safety, and railroad policy in Florida.